A blood exam to position the presence of tummy ulcers inwards horses is a footstep closer afterward researchers identified biomarkers linked to the disease.
Stomach ulcers are mutual inwards horses. They tin dismiss acquit on the upper non-acid-producing division of the stomach, where it is known every bit Equine Squamous Gastric Disease (ESGD), too the lower acid-producing glandular tissue, where it is known every bit Equine Glandular Gastric Disease (EGGD).
The electrical flow gilded criterion for diagnosing too monitoring tummy ulcers is gastroscopy, only it is non suited to screening every bit it is time-consuming too requires expensive equipment.
A less invasive too cheap method would hold upwardly preferred, according to researchers.
Parichart Tesena too her colleagues focused their inquiry on EGGD, which is the equivalent of peptic ulcers inwards humans.
The researchers noted that at that spot were screening tests available – a commercial blood fecal exam too sucrose permeability exam – only their specificity is depression too they cannot distinguish betwixt ESGD too EGGD.
The study focused on v Thoroughbred horses aged 8 to 12 years amongst a history of lameness only no obvious gastrointestinal problems. The animals had a normal appetite, their feces appeared normal, too they had no tape of whatever other sickness for to a greater extent than than ii years.
The horses were given high doses of the nonsteroidal anti-inflammatory drug phenylbutazone (bute), which is oftentimes given for lameness, for ii days. The dose was too therefore reduced to conventional levels.
Gastroscopies showed that none of the horses had EGGD earlier receiving the high dose of phenylbutazone. All developed tummy ulcers inwards the lower acid-producing division of their tummy every bit a lawsuit of receiving the drug. After handling amongst omeprazole for a month, the tummy lining was repaired inwards all horses.
Blood was taken from each of the horses for analysis during the 3 phases of the experiment – earlier they were given phenylbutazone, during the stage when they had developed the ulcers, too a calendar month afterward they had gone on to the anti-ulcer treatment.
The study team, writing inwards the Journal of Veterinary Medical Science, reported that their serum poly peptide analysis identified fourteen proteins that mightiness hold upwardly used every bit EGGD markers.
“However, confirmation of these candidate marking proteins is required amongst specific antibodies inwards the larger Equus caballus population earlier they tin dismiss hold upwardly considered for application inwards the field,” they wrote.
The study squad comprised Parichart Tesena, amongst Chulalongkorn too Mahidol universities inwards Thailand; Yodying Yingchutrakul too Sittiruk Roytrakul, amongst the National Center for Genetics
Engineering too Biotechnology; Tuempong Wongtawan, amongst Mahidol University; too Kris Angkanaporn, amongst Chulalongkorn University.
Serum poly peptide facial expression inwards Equine Glandular Gastric Disease (EGGD) induced past times phenylbutazone
Parichart Tesena, Yodying Yingchutrakul, Sittiruk Roytrakul, Tuempong Wongtawan too Kris Angkanaporn.
J. Vet. Med. Sci. 81(3): 418–424, 2019 doi: 10.1292/jvms.18-0679