Differences in gene expression in embryos seen when frozen semen is used

By | June 25, 2019

Genes relevant for early on embryo evolution inwards horses are downwardly Differences inwards cistron aspect inwards embryos seen when frozen semen is used

Genes relevant for early on embryo evolution inwards horses are down-regulated when frozen semen is used, researchers bring found.

Down-regulation is the physical care for yesteryear which a prison theatre cellphone decreases the quantity of a cellular component, such every bit RNA or protein, which are coded yesteryear the genes.

Fernando Peña in addition to his colleagues, writing inwards the open-access periodical PLOS ONE, acknowledged the preliminary in addition to descriptive nature of their report.

“But our information supply strong prove that cryopreservation of sperm exerts a profound touching on on the transcriptome (the prepare of all RNA molecules) of early on embryos.

“As sperm cryopreservation is usually used inwards many species, including human, the final result of this intervention on aspect of developmentally of import genes inwards resulting embryos warrants attention,” they said.

The researchers said artificial insemination amongst cryopreserved spermatozoa – to a greater extent than usually called frozen semen – is a major assisted reproductive technology scientific discipline inwards many species.

They said the contribution of sperm to embryo evolution has evolved from the concept that the solely role of sperm at fertilization is to innovate the manly mortal genome into the egg.

Sperm, they said, behave a myriad of modest noncoding RNAs amongst potential roles inwards early on embryo development.

In horses, every bit inwards humans, insemination amongst cryopreserved sperm is associated amongst lower pregnancy rates than those for fresh sperm, suggesting that preservation methods are currently sub-optimal. It leads to harm to sperm prison theatre cellphone membranes in addition to causes metabolic in addition to functional change of sperm, especially of their mitochondria.

However, the straight effects of sperm cryopreservation on the evolution of resulting embryos are largely unexplored, they noted.

In a study based at the University of Extremadura inwards Spain, scientists prepare out to investigate differences inwards cistron aspect betwixt embryos resulting from fertilization amongst fresh or cryopreserved sperm.

Embryos were obtained at 8, 10 or 12 days afterward ovulation from mares inseminated on successive cycles amongst either fresh sperm or frozen-thawed sperm from the same stallion, effectively providing matched embryo pairs at each day.

RNA was isolated from 2 matched pairs (4 embryos) for each twenty-four hours for analysis.

The transcriptional profile – cistron aspect at the RNA score – of embryos obtained amongst frozen-thawed sperm differed significantly from that for embryos derived from fresh sperm on all days, showing pregnant down-regulation of genes involved inwards biological pathways related to oxidative phosphorylation (an of import machinery yesteryear which the activeness of proteins tin travel altered afterward they are formed), deoxyribonucleic acid binding, deoxyribonucleic acid replication, in addition to immune response.

The authors say their piece of occupation provides the get-go prove of altered transcription inwards embryos resulting from fertilization amongst frozen semen inwards whatsoever species.

Discussing their findings, the study squad said the prove showed that procedures performed during the treatment of sperm, such every bit freezing in addition to thawing, bring a pregnant touching on on critical aspects of the early on embryo transcriptome.

“While the mechanisms behind the effects reported hither are every bit soundless unclear, a major factor may travel the well-documented oxidative harm that the genome in addition to epigenome experiences during cryopreservation in addition to thawing.” Cryopreservation is a major drive of oxidative stress in addition to lipid peroxidation (degradation) inwards stallion sperm, they said.

The sum study squad comprised Peña, José Ortiz-Rodriguez, Cristina Ortega-Ferrusola, María Gil, Francisco Martín-Cano, Gemma Gaitskell-Phillips, Alberto Álvarez-Barrientos, in addition to Ángel Román (all amongst the University of Extremadura), in addition to Heriberto Rodríguez-Martínez (Linköping University inwards Sweden), in addition to Katrin Hinrichs (Texas A&M University).

Ortiz-Rodriguez JM, Ortega-Ferrusola C, Gil MC, Martín-Cano FE, Gaitskell-Phillips G, Rodríguez-Martínez H, et al. (2019) Transcriptome analysis reveals that fertilization amongst cryopreserved sperm downregulates genes relevant for early on embryo evolution inwards the horse. PLoS ONE 14(6): e0213420. https://doi.org/10.1371/journal.pone.0213420

The study, published nether a Creative Commons License, tin travel read here


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